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KMID : 0385520080210060535
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Analytical Science & Technology
2008 Volume.21 No. 6 p.535 ~ p.542
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Analysis of clenbuterol in bovine muscle and milk by LC-ESI/MS/MS
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Hong Se-Lyung
Jeong Ji-Yoon
Park Hye-Jin
Lee Soon-Ho
Lee Jong-Ok
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Abstract
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A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) method was developed for the determination and confirmation of clenbuterol in bovine muscle and milk. Clenbuterol and clenbuterol-D9 using as an internal standard in samples were extracted with ethyl acetate after hydrolysis and evaporated to dryness. The extracts were dissolved in 20% methanol and cleaned using HLB solid-phase extraction cartridge. The analytes were detected by LC-ESI/MS/MS on a C₁? column. Mass spectral acquisition was done in selected reaction monitoring (SRM) in positive ion mode to provide a high degree of sensitivity. Using MS/MS with SRM mode, the transitions (precursor to product) monitored were m/z 277→203 for clenbuterol, and m/z 286→204 for internal standard. The limits of quantitation (LOQ) and mean recoveries of clenbuterol in bovine muscle were 0.2 μg/kg and 84.3~91.1%, respectively. The LOQ and mean recoveries in milk were 0.05 ㎍/㎏ and 87.7~98.3%, respectively.
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KEYWORD
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clenbuterol, LC-ESI/MS/MS, bovine muscle, milk
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